7703 Floyd Curl Drive San Antonio, TX 78229

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Bo Huang, PhD
Dept. of Pharmaceutical Chemistry
Dept. of Biochemistry and Biophysics
University of California, San Francisco

Title: "Mapping the inner world of cells"

Summary: Cellular processes are orchestrated by a large number of biomolecules in a spatially and temporally coordinated manner within a tiny volume. To uncover the underlying organizational principles and their functional relevance, we take microscopy visualization as the primary approach to systematically map their spatial localization, temporal dynamics and activity profiles. By combining small tags engineered from split fluorescence proteins and CRISPR/Cas9-mediated gene editing, we have enabled large-scale tagging of endogenous proteins in human cell lines for both microscopy visualization and biochemical analysis. Characterization of their live cell dynamics using epi-illumination light-sheet microscopy reveals intriguing phase condensation and decondensation behavior of many proteins during the cell cycle. We have further developed the deep-learning framework to connect cellular images of proteins to their amino acid sequences.

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